"Indole derivatives interact with DOPC in Langmuir monolayers"
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Authors
Brozik, Melissa
Frazee, Erin
Bohorquez, Maria
Issue Date
2005-06-28T19:03:07Z
Type
Presentation
Language
en_US
Keywords
Fluorescence , Monolayers , Phospholipids , Amino acids
Alternative Title
Abstract
Molecular interactions between indole derivatives and 1,2-di-oleoyl-sn-glycero-3-phosphocholine (DOPC) were studied using a Nima Langmuir-Blodgett (LB) trough. A 0.25 M phosphate buffer sub-phase was used with a physiological pH range of 7.31-7.48. The air-phosphate buffer interface was examined at room temperature. Constant ionic strength was maintained by 0.100 M potassium chloride (KCl). Two different indole derivatives were used, indole and tryptophan, in concentrations of 3.0 uM and 5.0 uM. It is evident when examining the isotherms that the DOPC monolayer is expanded in the presence of indole derivatives, as the area per molecule was increased by 0.02nm2/molecule.
Description
Melissa Brozik and Erin Frazee are students at Drake University. Maria Bohorquez is Associate Professor of Chemistry in the College of Arts and Sciences at Drake. She can be contacted at maria.bohorquez@drake.edu