The Relationship Between Resistance to the Fungal Toxin Cercosporin and Grey Leaf Spot Disease Resistance in Corn (ZEA MAYS L.)

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Authors

Lair, Bradley S.

Issue Date

1989-12

Type

Thesis

Language

en_US

Keywords

Corn disease and pest resistance , Mycotoxins

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Abstract

The problem. Corn is infected by the fungus "Cerospora zeae-maydis" which causes the disease Grey Leaf Spot. The fungus produces a toxin, cercosporin, whose role in the pathogenicity of the disease is not known. The first step in determining the importance of the toxin to the fungus is to screen corn inbreds to see if any disease resistant inbreds are also resistant to the toxin. Procedure. Thirteen corn inbreds were tested for their degree of resistance to the fungal toxin cercosporin. The level of tolerance to cercosporin for each inbred was then compared to the inbred's level of resistance to Grey Leaf Spot. Resistance to cercosporin was determined by measuring the increases in the conductivity of water containing leaf tissue slices following exposure to cercosporin. Ion leakage is an indicator of cellular damage, so resistant tissue would be expected ro have a lower level of conductivity increase than susceptible tissue. Resistance to Grey Leaf Spot disease had been previously determined by field test conducted by Pioneer Hi-Bred International, Inc. Findings. The ion leakage assay showed that the inbreds which were more disease resistant tended to have a lower tolerance for cercosporin than did the less disease resistant inbreds (correlation coefficient = 0.5524). Conclusion. Based on the lack of a positivc relationship between disease susceptibility and cercosporin susceptibility it was concluded that cercosporin resistance does not have a significant role in the prevention of Grey Leaf Spot infections among the tested inbreds.

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65 leaves. Advisor: Michael E. Myszewski

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Drake University

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