Expression of Plant Receptor-Like Kinase Leucine Rich Repeat Regions in E. Coli
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Authors
Corns, Andrea
Heffernen, Emily
Hubbard, Ara May
Macksey, Paula
Pigg, Kelsey
Platt, Andrew
Stieff, Maria
Tucci, Julia
Issue Date
2011-04-19T16:17:16Z
Type
Presentation
Language
en_US
Keywords
Arabidopsis , Bacteria , Leucine , Ligands
Alternative Title
Abstract
Plants respond to external stimuli through signal transduction pathways. An external signal binds the
extracellular domain of a transmembrane receptor-like kinase (RLK) protein, and the signal is transmitted
inside the cell through the RLK’s kinase domain. Two important plant RLKs are Arabidopsis
CLV1 and rice Xa21. CLV1 regulates plant meristem growth and development, and Xa21 provides
resistance to the pathogenic bacterium Xanthomonas oryzae. These functions are initiated by a peptide
ligand binding a leucine-rich repeat (LRR) region in the extracellular domains of CLV1 and
Xa21. Peptide phage display technology will be used to isolate additional peptide ligands that bind
CLV1 and Xa21 LRRs. To this end, the DNAs encoding these LRRs are being cloned into vector
pSUMO_Kan for soluble expression in E. coli as a fusion with SUMO protein. Fusion proteins will
be isolated from E. coli cells by immobilized metal affinity chromatography.
Description
Mentor: Heidi Sleister