Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells
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Authors
Steger, Ann M.
Issue Date
1997-09
Type
Thesis
Language
en_US
Keywords
Swine--Cells--Development , Swine--Cells--Evaluation , Testis--Cells--Analysis
Alternative Title
Abstract
An in vitro Isospora suis sporozoite neutralizing antibody assay has been developed
in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies
present in sera, milk and monoclonal antibody culture fluids by determining both the
amount of intracellular sporozoites by immunofluorescence and the cytopathic effect in ST cells. A monoclonal antibody against the apical complex of I. suis sporozoites inhibited the infection of sporozoites in vitro. Hyperimmune sera produced in goats and guinea pigs inhibited sporozoite infection in ST cells. Milk samples from gilts vaccinated with I. suis contained neutralizing antibodies, however the titers did not correlate with protection observed in nursing pigs after challenge.
Description
41 leaves
Citation
Publisher
Drake University