The Effect of Leucogenenol on Classical Chemotherapy and Immunotherapy of Friend Virus Disease
Muckerheide, Annette, Sister
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The problem. These experiments determined the effects of the immunostiumulants leucogenenol and Bacille Calmette-Guerin (BCG) in combination with Methotrexate chemotherapy on Friend Virus Disease. Procedure. FV infected mice were treated with various combinations of the chemo-and immunotherapeutic agents being investigated. Three parameters of the disease, survival time, ratios of spleen and liver weight to total body weight and periodic differential counts of peripheral blood were studied and analyzed. Findings. Although Methotrexate significantly prolonged survival time in animals thus treated, the other parameters examined were not significantly different from the untreated controls at the time of death. Immunostimulation with leucogenenol, BCG, or both had no appreciable effect on any of the parameters of the disease. Conclusion. It was concluded that the effect of MTX therapy was to suppress the production and sensitization of T-lymphocytes so efficiently that consequent attempts at restimatulation were ineffective. Furthermore, this suppression was sufficiently great so that upon cessation of MTX therapy the proliferation of leukemia cells probably exceeded that of new, immunocompetent T-lymphocytes. Recommendations. More studies on the molecular and cellular action of leucogenenol are necessary. An important question raised by this study is which kind of lymphocytes are stimulated by leucogenenol. If only B-lymphocytes are affected, the overall result may be to enhance tumor growth by a blocking effect on the tumor receptor sites. Further, leucogenenol should be tried as therapy against a solid, localized tumore system which does not directly affect the hemotopoietic process.