Protein-Protein Interactions in Drosophila Germ Cell Development
MetadataShow full item record
During germ cell formation in Drosophila embryos, cells migrate from their site of origin to the site of organ formation. In particular, germ cells need to migrate long distances from their site of origin to the site where ovaries and testicles develop. It was found that the this cell migration is guided by a chemical signaling pathway. Cells which digress from the migration route are destroyed by apoptosis. Dr. Coffman's group at Iowa State University has identified mutations that impair the destruction of cells that have strayed from their migration path. One of these mutations affects the function of a G-protein coupled receptor, Trel. In this study we look at physical interactions of the Trel protein with ten candidate proteins. We have identified interactions between the G-protein coupled receptor Trel and two, possibly three, candidate proteins. These candidates are members of a protein phosphatase family that have previously been implicated in germ cell migration, which makes our result particularly valuable. Adding to the picture is the fact that the phenotypes of mutations in the corresponding genes closely resemble trel mutant phenotypes. All of the above results indicate that these proteins function in the same pathway.
Showing items related by title, author, creator and subject.
Identifying Human Host Proteins That Interact With Influenza A Polymerase Proteins Using The Yeast Two-Hybrid System Drew, Cassandra; Faganel, Peyton; Johnson, Bree; Kinzler, Carly; Madhan, Nayasha; Schmotter, Caleb; Tourville, Luke; Rose, Alec; Marshall, Kevin; Busch, Marc; Scully, Erin (2013-04-16)Influenza viruses are enveloped, single-stranded RNA viruses which cause 250,000 to 500,000 deaths per year. Understanding how influenza A viral proteins interact with human proteins is essential for identifying factors ...
Gilbreath, Michael Joye (Drake University, 1973-09)The problem. Isolation of Friend virus protein in an increased yield for immunological purposes by zonal centrifugation was the purpose of this experiment. Procedure. Sucrose gradient zonal centrifugation was used to ...
Andrews, Brian T. (Drake University, 1998-06)The cell cortex (or pellicle) of "Tetrahymena" is composed of three closely associated layers: the cell membrane, the alveolar sacs, and the epiplasm. The epiplasm is the innermost layer of the cortex and is thought to ...