The Expression of Endothelin Converting Enzyme-1 Insoforms in Murine Cells

DSpace/Manakin Repository

Show simple item record Chawla, Kashmira 2009-06-24T16:26:43Z 2009-06-24T16:26:43Z 2009-06-24T16:26:43Z
dc.description Advisor: Andrew Brittingham ; Jeffrey Divino of Des Moines University en_US
dc.description.abstract Endothelin-1 (ET-1) has been demonstrated to play a role in many physiological processes including vasoconstriction, cardiovascular homeostasis, inflammation, angiogenesis, and tissue regeneration. A critical step in the production of ET-1 is the conversion of the propeptide form, bigET-1, to ET-1 via the action of endothelin converting enzyme-1 (ECE-1). There are four major ECE-1 isoforms: ECE1a, 1b, 1c, and 1d, which differ only in their NH2 terminus and subcellular locations. Here we describe our analysis of the expression of ECE-1 isoforms in murine bone marrow derived macrophages (BMM0), macrophage-like cell lines (RAW264, J774) and an endothelial cell line (bEND.3). For analysis, reverse transcription-polymerase chain reaction (RT-PCR) was performed using forward primers, unique to each major isoforms, and a common reverse primer. bEnd.3, J774 and BMM0 express mRNA encoding all four major isoforms of ECE-1, as well as previously reported splice variants (SV) of ECE-1b, 1c, and 1d. A novel splice variant, KC150, was identified in isoform 1c. These splice variants all lack exon 3, which encodes the trans-membrane domain of ECE-1. The absence of a trans-membrane domain could be indicative of a secreted or cytosolic form of ECE-1. Our results also indicate that the RAW264 macrophage cell line expresses very low levels of ECE-1 encoding mRNA. This verifies our previous finding that RAW264 cells secrete only bigET-1, suggesting a deficiency in ECE-1 expression. We hope to use these ECE-1 deficient cells as a model for over expressing individual ECE-1 isoforms and splice-variants, and examine their function in regulating macrophage ET-1 production. en_US
dc.description.sponsorship Drake University, College of Arts and Sciences, Department of Biology, Biochemistry, Cell and Molecular Biology Program ; Department of Microbiology, Des Moines University en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2009;34
dc.subject Enzymes en_US
dc.subject Physiology en_US
dc.subject Neovascularization en_US
dc.subject Inflammation en_US
dc.title The Expression of Endothelin Converting Enzyme-1 Insoforms in Murine Cells en_US
dc.type Presentation en_US

Files in this item

This item appears in the following Collection(s)

  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

Show simple item record

Search DSpace

Advanced Search


My Account