Caspase-Dependent Cleavage of an Immediate Early Herpes Simplex Virus Protein

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dc.contributor.author Craig, Trevor
dc.contributor.author Pradhan, Prajakta
dc.date.accessioned 2009-04-22T16:37:45Z
dc.date.available 2009-04-22T16:37:45Z
dc.date.issued 2009-04-22T16:37:45Z
dc.identifier.uri http://hdl.handle.net/2092/899
dc.description Advisor: Marie Nguyen of Des Moines University en_US
dc.description.abstract Herpes Simplex Virus 1 (HSV-1) is a member of the Herpesviridae family. All herpesviruses cause life-long infections that shift between latent and lyticand have many treatments but no cures. HSV-1 has a double-stranded, linear DNA genome, stages icosahedralcapsid, and envelope. HSV-1 can cause infections in humans ranging from encephalitis (brain Inflammation) to cold sores. Previous studies have determined that apoptosis is triggered early during HSV-1 infection. A necessary event in the induction of apoptosis is the activation of a specific set of cellular proteases called caspases. The goal of this study was to determine whether specificviral proteins were cleaved by these caspases. During the course of infection the virus expresses different proteins classified as Immediate Early, Early, and Late proteins. These experiments focused on the Immediate Early proteins, ICPO, ICP4 and ICP27, that regulate expression of the early and late viral genes and are required for virus replication. A late viral protein, VP22, was also analyzed. Human carcinoma Hep-2 cells were used for infection and western blots were used to detect the viral protein. Here, we identify the presence of a low molecular weight form of the ICP27 protein in HSV-1 infected cells. The levels of this small form of ICP27 are undetectable when infections are performed in the presence of pan-caspase inhibitors. Together, these results suggest that ICP27 is cleaved in a caspase-dependent manner during infection. Future studies are aimed to confirm this result and elucidate the biological relevance of ICP27 cleavage. en_US
dc.description.sponsorship Drake University, College of Arts and Sciences, Department of Biology, Biochemistry, Cell and Molecular Biology Department ; Department of Microbiology and Immunology, Des Moines University en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2009;39
dc.subject Herpes simplex virus en_US
dc.subject Infections en_US
dc.subject Apoptosis en_US
dc.subject Proteins en_US
dc.title Caspase-Dependent Cleavage of an Immediate Early Herpes Simplex Virus Protein en_US
dc.type Presentation en_US


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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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