Isolation of Yeast Genes that Suppress the Chromosome Loss Defect of ysm's 22, 77, 83, and 84

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Show simple item record Carter, Chelsea Christie, Allen Dicken, Matthew Helget, Holly Hong, Davy Kim, Yun Mazurek, Stefan Michaels, Whitney Nguyen, Lahn G. Peterson, Whitney Swehla, Kendra Swenson, Jill Thomas, Maggie Tolliver, Clare Wilt, Nicole 2009-04-21T14:08:23Z 2009-04-21T14:08:23Z 2009-04-21T14:08:23Z
dc.description Advisor: Heidi Sleister en_US
dc.description.abstract Cancer cells display both chromosome rearrangements and abnormal numbers of chromosomes. High fidelity chromosome transmission requires the accuracy of multiple processes such as DNA replication, DNA repair, sister chromatid cohesion, mitotic spindle assembly, and chromosome segregation. Many of the gene products that function in these processes are conserved in eukaryotes. The simple eukaryote Saccharomyces cerevisiae (budding yeast) is an excellent model system for the study of chromosome transmission as its cell cycle is well-characterized, and it is amenable to genetic analysis. With the aim of identifying genes important for chromosome transmission, we previously generated a collection of YAC stability in mitosis (ysm) mutants that display increased loss of a yeast artificial chromosome (YAC). Four mutant strains (ysm's 22, 77, 83, 84) are particularly interesting as they display high levels of YAC loss and have at least one additional mutant phenotype related to a process important for genome stability (e.g., DNA replication, mitotic spindle assembly). Isolation of suppressors of the YAC loss phenotype in these mutants may reveal the defective genes in these strains as well as genes whose products interact in the same or parallel pathways as the defective gene product. To this end, ysm's 22, 77, 83, and 84 were transformed with a yeast genomic plasmid library, and plasmids that suppressed the YAC loss phenotype were isolated. We are in the process of verifying candidate suppressor plasmids by retransformation of the mutant strains and identifying the suppressing genes by DNA sequencing. Analysis of these suppressors will contribute to our understanding of gene products, protein networks, and processes important for eukaryotic genome stability. en_US
dc.description.sponsorship Drake University, College of Arts and Sciences, Department of Biology en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2009;6
dc.subject Mitosis en_US
dc.subject Yeast--Physiology en_US
dc.subject DNA en_US
dc.subject Chromosomes en_US
dc.subject Saccharomyces cerevisiae en_US
dc.title Isolation of Yeast Genes that Suppress the Chromosome Loss Defect of ysm's 22, 77, 83, and 84 en_US
dc.type Presentation en_US

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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