Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Bush, Paul J.
dc.date.accessioned 2008-10-03T16:37:40Z
dc.date.available 2008-10-03T16:37:40Z
dc.date.issued 1984-05
dc.identifier.other 1984 .B963
dc.identifier.uri http://hdl.handle.net/2092/797
dc.description 64 leaves. Advisor: Dr. Dean Hoganson en
dc.description.abstract The purpose of this project was to determine whether a chemiluminescent detection system can give the sensitivity of 32P isotopic detection for human DNA restriction fragment length polymorphism (RFLP) analysis and have the speed, safety, and reduced cost of colorimetric detection. The assessment was made on the basis of: cost, sensitivity, time of operation, ease of operation, and ease of multiple probings. Human DNA was extracted from whole blood and processed for RFLP analysis according to the December 1990 FBI Protocol: "Procedures for the Detection of Restriction Fragment Length Polymorphisms in Human DNA." Both the "Boehringer Mannheim Genius" system which uses digoxigenin labeled probes detected with alkaline phosphatase-conjugated anti-digoxigenin antibody, and the "Promega Gene Print Light" system which uses alkaline phosphatase bound directly to the probe, were examined. Probes pH30, YNH24, and TBQ7 were used. Visualization was with "Lumi-Phos" 530 and Kodak XAR X-ray film. Results show that chemiluminescent detection is better than colorimetric or 32P detection in all aspects but sensitivity. en
dc.format.extent 4826149 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Drake University en
dc.relation.ispartofseries Drake University, College of Arts and Sciences;1984
dc.subject DNA--Analysis en
dc.subject Chemiluminescence en
dc.subject Chromosome polymorphism en
dc.title Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis en
dc.type Thesis en


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account