Immunogenicity of Staphylococcus Aureus Antigens Against Experimentally Induced Mastitis in the Mouse and Optimization of Antigen Production in Fermenter Culture

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dc.contributor.author Edwards, Charlie R.
dc.date.accessioned 2007-09-26T19:18:42Z
dc.date.available 2007-09-26T19:18:42Z
dc.date.issued 1985-05
dc.identifier.other 1985 .Ed95
dc.identifier.uri http://hdl.handle.net/2092/643
dc.description 69 leaves. Advisor: Dean Hoganson en
dc.description.abstract Capsular antigens of "Staphylococcus aureus" Smith and 2-8 strains were evaluated for immunogenicity in mice. Cultural conditions necessary for maximum expression of the antigens were also defined. Actively growing "S. aureus" Smith strain culture was used to challenge lactating GF-1 strain mice which had been vaccinated with various capsule antigen preparations. Capsule antigen production was evaluated using shaker flask and fermenter cultures. Immunogenicity evaluations using single vaccinations showed that semi-purified capsule preparations elicited a protective response against challenge. Mice vaccinated twice showed that oil and aluminum hydroxide adjuvanted capsule antigens protected against challenge, but DDA (dimethyldioctadecylammonium bromide) adjuvanted capsule antigens resulted in more mastitis than non-vaccinated controls. Cultural evaluations showed that media high in nitrogen and carbohydrate content and grown at pH 5.5 resulted in the greatest amount of capsule antigen production. Capsular antigens may be more useful in preventing "S. aureus" mastitis than whole cell preparations. Media containing high concentrations of nitrogen source and carbohydrate are necessary to obtain large amount of capsular antigen. A culture pH of 5.5 promotes the production of additional antigens not found when grown at higher or lower pH levels. en
dc.format.extent 5080444 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Drake University en
dc.relation.ispartofseries Drake University, School of Graduate Studies;1985
dc.subject Staphylococcus aureus infections en
dc.subject Mice--Physiology en
dc.subject Antigens en
dc.subject Immunogenetics en
dc.title Immunogenicity of Staphylococcus Aureus Antigens Against Experimentally Induced Mastitis in the Mouse and Optimization of Antigen Production in Fermenter Culture en
dc.type Thesis en


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