Immunogenicity of Staphylococcus Aureus Antigens Against Experimentally Induced Mastitis in the Mouse and Optimization of Antigen Production in Fermenter Culture
Edwards, Charlie R.
MetadataShow full item record
Capsular antigens of "Staphylococcus aureus" Smith and 2-8 strains were evaluated for immunogenicity in mice. Cultural conditions necessary for maximum expression of the antigens were also defined. Actively growing "S. aureus" Smith strain culture was used to challenge lactating GF-1 strain mice which had been vaccinated with various capsule antigen preparations. Capsule antigen production was evaluated using shaker flask and fermenter cultures. Immunogenicity evaluations using single vaccinations showed that semi-purified capsule preparations elicited a protective response against challenge. Mice vaccinated twice showed that oil and aluminum hydroxide adjuvanted capsule antigens protected against challenge, but DDA (dimethyldioctadecylammonium bromide) adjuvanted capsule antigens resulted in more mastitis than non-vaccinated controls. Cultural evaluations showed that media high in nitrogen and carbohydrate content and grown at pH 5.5 resulted in the greatest amount of capsule antigen production. Capsular antigens may be more useful in preventing "S. aureus" mastitis than whole cell preparations. Media containing high concentrations of nitrogen source and carbohydrate are necessary to obtain large amount of capsular antigen. A culture pH of 5.5 promotes the production of additional antigens not found when grown at higher or lower pH levels.
Showing items related by title, author, creator and subject.
Antigenic Relationship of Different Porcine Rotavirus Isolates and the Identification of a New Porcine Strain Welter, Mark W. (Drake University, 1983-08)The problem. Currently, only one strain of porcine rotavlrus has been reported. This strain, designated OSU, has been attenuated and is currently available as a federally licensed, live oral vaccine for pigs. This study ...
Phenotypic Suppression of "Escherichia coli" K88ac antigen by selective media and termperature variations Jacob, Carl R. (Drake University, 1980-09)The K88ac antigen, thought to he a pilus on "Escherichia coli", has been determined to be one of the virulence factors in neonatal porcine colibacillosis. Using five different "E. coli" strains, the suppression of this ...
A Physical, Cultural, and Nutritional Study of "Leptospira Interrogans" Serotype "Hardjo" with Emphasis on Antigenicity, Immunogenicity, and Viability Mickle, Thomas Richard (Drake University, 1983-08)The problem. "Leptospira interrogans" serotype "hardjo" has been indicated as the cause of abortion and clinical leptospirosis in cattle. It is a fastidious organism and has been difficult to reisolate from infected ...