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dc.contributor.authorZentrich, Eve
dc.date.accessioned2007-02-02T15:33:47Z
dc.date.available2007-02-02T15:33:47Z
dc.date.issued1993-07
dc.identifier.other1993 .Z46
dc.identifier.urihttp://hdl.handle.net/2092/508
dc.description[iv], 57 leaves. Advisor: Russell Sextonen
dc.description.abstractBovine aortic smooth muscle cells (BASMC) in culture have been shown to oxidatively modify human low-density lipoprotein (LDL). Such oxidized LDL appears to be a significant factor in the etiology of atherosclerosis. This work shows that the azole antifungal drug ketoconazole when added to the culture medium of BASMC inhibits LDL oxidation. Confluent BASMC were fed 1 ml of F- 12/DMEM containing LDL (320 ug protein). The cells over a 24 hour period caused a 2x increase in LDL oxidation as measured by an increase in thiobarbituric acid reactive substances. Copper at 5 uM enhanced by 2x the oxidation of LDL by BASMC. Oxidized LDL was found to be cytotoxic to the BASMC. Addition of ketoconazole to the cell medium caused a concentration dependent inhibition of LDL oxidation by BASMC. Maximum inhibition of LDL oxidation was achieved at 20 uM of ketoconazole. BASMC viability was maintained in the presence of ketoconazole and LDL as determined by the protein content per well.en
dc.format.extent2414647 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherDrake Universityen
dc.relation.ispartofseriesDrake University Theses, College of Arts and Sciences;1993
dc.subjectLow density lipoproteins--Oxidationen
dc.subjectKetoconazole--Effectivenessen
dc.subjectMuscle cellsen
dc.subjectVascular smooth muscleen
dc.titleThe Effects of Ketoconazole on the Oxidation of Low Density Lipoproteins by Bovine Aortic Smooth Muscle Cellsen
dc.typeThesisen


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