Characterization of the Surface Proteins of the Anatid herpesvirus

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Show simple item record Sesslar, Lee Christine 2006-12-12T14:38:11Z 2006-12-12T14:38:11Z 1995-08
dc.identifier.other 1995 .S73
dc.description [v], 48 leaves. Advisor: James C. Johnson en
dc.description.abstract A plaque purified isolate, Anatid herpesvirus (AHV-ppc3) of the Holland strain of AHV (American Type Culture Collection No. VR684) was propagated in chicken embryo fibroblasts and purified by differential and buoyant density sedimentation through thtough sucrose gradients. A total of 17 putative virus proteins were consistently identified in both polyacrylamide gel electrophoresis and radioautogram. Coomassie blue staining techniques identified 10 virus bands. The remaining 7 virus proteins were identified using radioautography. AHV polypeptides identified ranged from 6 to 199 Kd. AHV contains major polypeptides with molecular weights of 173, 144, 118, 105, and 76.3 Kd. In addition, AHV possess minor polypeptide bands with molecular weights of 195, 183, 92.8, 81.4, 55.7, 52.0, 42.2, 32.6, 17.1, 7.91, and 6.16 Kd. It has been demonstrated, in this study, that the Anatid herpesvirus putative surface proteins are very similar in molecular weights to those reported for other herpesviruses. en
dc.format.extent 2513420 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Drake University en
dc.relation.ispartofseries Drake University Theses, College of Arts and Sciences;1995
dc.subject Herpesviruses en
dc.subject Proteins en
dc.subject Peptides en
dc.subject Duck plague en
dc.subject Duck plague virus en
dc.title Characterization of the Surface Proteins of the Anatid herpesvirus en
dc.type Thesis en

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