Basal Release of Endothelium-derived Nitric Oxide in Thyropathologic Rat Aorta

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Show simple item record Illig, Lisa C. Frazzell 2006-10-25T13:23:26Z 2006-10-25T13:23:26Z 1994-01
dc.identifier.other 1994 .F869
dc.description 35 leaves. Advisor: Donald B. Stratton en
dc.description.abstract Systolic blood pressures, basal metabolic rates, and circulating catecholamine levels were determined for hyperthyroid (TRX), hypothyroid (PTU), and euthyroid control (CON) rats. All three measurements were elevated in TXX animals, while PTU rats were determined to have decreased measurements when compared with CON and TRX rats. Animals were sacrificed by either cervical dislocation or pentobarbital injection followed by thoracic opening to test for sacrifice differences. Three aortic rings from each rat were mounted in environmentally-controlled tissue baths and contracted with different concentrations (10 nM, 100 nM, and 1 mM) of phenylephrine (PE), an alpha-1 adrenoreceptor agonist. At steady state, methylene blue (MB) (10 mM), an endotheliumderived nitric oxide (EDNO) inhibitor, was added and rings were allowed to contract further. At steady state, a high PI3 dose (10 mM) was added to produce maximum contraction. Irrespective of the sacrifice method, the basal release of EDNO as a percent of the maximum force generated, was PTU>CON>TRX, while the mg of force unmasked by MB was not different. The trend in mg of force produced by PE was TRX>CON>PTU regardless of the initial PE concentration. As the concentration of the inital PE dose was increased, the percent of the total PE plus ME3 response that was due to MB alone decreased in CON rats. Sacrifice with pentobarbital followed by thoracic opening eliminated the difference between TRX and CON expressed in animals sacrificed by cervical dislocation, while the relationship between CON and PTU was unchanged. Just as thyropathology differentially altered systolic blood pressure, basal metabolic rare, and circulating catecholamine levels, the basal release of EDNO in rat aorta was found to be dependent upon the thyroid state of the animal and the initial agonist-induced tone. en
dc.format.extent 1682415 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Drake University en
dc.relation.ispartofseries Drake University Theses, College of Arts and Sciences;1994
dc.subject Drugs--Metabolism en
dc.subject Cardiovascular system--Research en
dc.subject Heart--Physiology en
dc.subject Blood pressure--Measurement en
dc.subject Thyroid gland--Diseases en
dc.subject Rats as laboratory animals en
dc.title Basal Release of Endothelium-derived Nitric Oxide in Thyropathologic Rat Aorta en
dc.type Thesis en

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