"Implementation of a genetic screen to elucidate proteins important for chromosome transmission"

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dc.contributor.author Bjordahl, Ryan
dc.contributor.author Davison, Rob
dc.contributor.author Hatz, Daniel
dc.contributor.author Kostelc, Kate
dc.contributor.author Larew, Robbie
dc.contributor.author Miller, Adam
dc.contributor.author Rusdianto, Eveline
dc.contributor.author Sawaya, Elizabeth
dc.contributor.author Sleister, Heidi
dc.date.accessioned 2006-06-07T16:10:02Z
dc.date.available 2006-06-07T16:10:02Z
dc.date.issued 2006-06-07T16:10:02Z
dc.identifier.uri http://hdl.handle.net/2092/387
dc.description.abstract The process of cell division is highly regulated in eukaryotic cells. Failure to transmit chromosomes accurately during cell division can result in cell death or abnormal growth (e.g., tumors). Proteins that associate in multisubunit complexes required for chromosome replication, structure, and segregation (e.g. histones) are typically expressed in the cell at precise levels. Alteration of these levels could lead to inappropriate stoichiometries of the complex subunits, possibly resulting in an abnormal complex structure and/or function. Students in Drake University's BIO106: Research in Genetics course designed and implemented a genetic screen to identify genes important for chromosome transmission. This screen involves a visual assay of the segregation of a yeast artificial chromosome (YAC) in cells tranformed with high copies of yeast genes. An increased copy number of a gene is expected to be correlated with increased amounts of the protein product. Transformation of a YAC-containing yeast strain with a high copy yeast genomic library resulted in 12,625 transformants. A visual screen of these transformants for YAC instability indicated that 123 display higher levels of YAC loss than the control strain; 40 were severely defective in chromosome segregation. In addition to characterization of these high copy plasmid transformants, the specific yeast genes responsible for chromosome instability when present at high copy will be identified. This genetic screen is expected to elucidate proteins that are components of multisubunit complexes that play a role in chromosome transmission. en
dc.description.sponsorship Drake University, College of Arts and Sciences, Department of Biology. en
dc.format.extent 1068220 bytes
dc.format.mimetype image/jpeg
dc.language.iso en_US
dc.relation.ispartofseries DUCURS 2005;8
dc.subject Genetics. en
dc.subject Chromosomes. en
dc.subject Genes. en
dc.subject Genetic screening. en
dc.subject Genetics--Research. en
dc.title "Implementation of a genetic screen to elucidate proteins important for chromosome transmission" en
dc.type Presentation en

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  • DUCURS [193]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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