"Identification of yeast genes important for chromosome transmission"

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dc.contributor.author Leeson, Stephanie
dc.contributor.author Fatland, Sarah
dc.contributor.author Bellani, Denisa
dc.contributor.author Scolaro, Eric
dc.contributor.author Wood, Abby
dc.contributor.author Young, Chris
dc.contributor.author Sleister, Heidi
dc.date.accessioned 2006-05-05T15:48:01Z
dc.date.available 2006-05-05T15:48:01Z
dc.date.issued 2006-05-05T15:48:01Z
dc.identifier.uri http://hdl.handle.net/2092/363
dc.description.abstract Accurate transmission of chromosomes during cell division requires that chromosomes are fully replicated and free of damage prior to cell division. Furthermore, cellular processes that separate chromosomes must be functional. As part of an effort to identify proteins important for chromosome transmission, we implemented a genetic screen in the yeast Saccharomyces ceresisiae that allows for visual detection of mutants with an increase in the loss of an ADE2-marked yeast artificial chromosome (YAC). This screen resulted in 132 YAC stability in mitosis (ysm) mutants. In addition to genetic characterization of these mutants, subsets of the ysm's were analyzed for YAC loss rate, sporulation efficiency, forward mutation frequency, and cell morphology. Mutants were also tested for sensitivity to high salt, caffeine, hydroxyurea, and benomyl. The results elucidated mutant phenotypes that will facilitate cloning the wild-type genes that are altered in these mutants. To this end, high copy suppressors of ysm83 and ysm84 have recently been isolated. en
dc.description.sponsorship Drake University, College of Arts and Sciences, Department of Biology. en
dc.format.extent 1221437 bytes
dc.format.mimetype image/jpeg
dc.language.iso en_US
dc.relation.ispartofseries DUCURS 2005;6
dc.subject Yeast fungi--Genetics. en
dc.subject Fungi--Genetics. en
dc.subject Saccharomyces cerevisiae--Genetics. en
dc.subject Chromosomes. en
dc.title "Identification of yeast genes important for chromosome transmission" en
dc.type Presentation en


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  • DUCURS [193]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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