"Detection of PIGF protein in neonatal rat cardiomyocytes"
Torry, Ronald J.
Torry, Donald S.
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SubjectAngiogenesis; Placenta Growth Factor (PIGF); Vascular Endothelial Growth Factor (VEGF); Ischemic heart disease
Angiogenesis, the formation of blood vessels, can provide blood to the heart when its normal arteries are compromised. Angiogenesis is highly dependent on the Vascular Endothelial Growth Factor (VEGF) family. Placental Growth Factor (PlGF) is a member of this family and is crucial for pathological angiogenesis in the adult. PlGF coupled with the more common VEGF could greatly increase angiogenesis in the heart tissue, thus providing oxygen to ischemic heart tissue. Previous research has shown that PlGF mRNA increases with six hours of hypoxia which models ischemia. However, not much is known about PlGF expression on the protein level. We intend to establish that PlGF protein expression will increase in rat cardiomyocytes which have undergone 6, 12 and 24 hours of hypoxia compared to normoxic rat cardiomyocytes. This information will later be used in studies of potential protective treatments using PlGF.
Marin Schweizer, Kyle Bohman, Carrie Mittelstedter and Ben Colton are all Drake University students. Ronald J. Torry is Associate Professor of Pharmacology at Drake University. Donald S. Torry is on the faculty at Southern Illinois University School of Medicine.