"Protective effect of Placenta Growth Factor (PIGF) against hypoxia-reoxgyenation and serum-deprivation induced apoptosis in neonatal rat cardiomyocytes"

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dc.contributor.author Bohman, Kyle
dc.contributor.author Schweizer, Marin
dc.contributor.author Berendt, Bob
dc.contributor.author Torry, Ronald J.
dc.contributor.author Torry, Donald S.
dc.date.accessioned 2005-07-05T13:50:48Z
dc.date.available 2005-07-05T13:50:48Z
dc.date.issued 2005-07-05T13:50:48Z
dc.identifier.uri http://hdl.handle.net/2092/276
dc.description Kyle Bohman, Marin Schweizer and Bob Berendt are all Drake University students. Ronald J. Torry is Associate Professor of Pharmacology at Drake University. Donald S. Torry is a faculty member at the Southern Illinois University School of Medicine. en
dc.description.abstract Placenta growth factor (PlGF) is known to induce angiogenesis and protect placental trophoblast from apoptosis. We have shown that PlGF mRNA expression is increased in hypoxic human myocardium and in rat neonatal cardiomyocytes. However, little is known regarding the function of PlGF in heart tissue. Others have shown that PlGF or PlGF/VEGF significantly inhibited apoptosis in endothelial cells from PlGF knockout mice and we have shown that PlGF rescues cultured trophoblast from apoptosis induced by serum deprivation. Accordingly, our hypothesis is that PlGF protects cardiomyocytes from ischemia-induced or serum deprivation-induced apoptosis. The role of PlGF will be investigated during cardiomyocyte apoptosis induced by serum-deprivation and hypoxia-reoxygenation (H/R)--physiological conditions relevant to ischemic cardiomyopathy. Hypoxic conditions were established by culturing the cells at 1-2% O2. Caspase-3,7 luminescence assay (Promega) was used to determine the level of activated Caspases 3 and 7. The caspase family of cysteine proteases, especially caspase-3, is central in amplifying the cascade of proteolysis that culminates in cell death. Serum-deprivation and H/R have been found to be consistent methods of creating ischemic stresses and inducing apoptosis in rat cardiomyocytes. Serum-deprivation produced a 1.55 fold increase of apoptosis over normoxic values (n=10). H/R lead to a 1.98 fold increase of apoptosis (n=1). Our preliminary data suggest that a PlGF (25ng/ml) or PlGF/VEGF (25ng/ml each) does not reduce apoptosis induced by serum-deprivation. However, an 8 hour pretreatment of PlGF (25ng/ml) by itself reduced caspase 3 activity by 25.5% (n=1) and 30.7% (n=3) during hypoxia or serum deprivation, respectively. The preliminary data show an 8 hour pretreatment with PlGF/VEGF (25ng/ml each) decreased caspase activity more than PlGF (25ng/ml) alone. Thus, early evidence indicates that pretreatment with PlGF or PlGF/VEGF may protect cardiomyocytes from hypoxia-reoxygenation and/or serum deprivation-induced apoptosis. en
dc.description.sponsorship Drake University, College of Pharmacy and Health Sciences. Department of Pharmaceutical Sciences. en
dc.format.extent 1064345 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.relation.ispartofseries DUCURS 2005;26
dc.subject Apoptosis en
dc.subject Placenta Growth Factor (PIGF) en
dc.subject Hypoxia en
dc.subject Angiogenesis en
dc.subject Vascular Endothelial Growth Factor (VEGF) en
dc.subject Ischemic heart disease en
dc.title "Protective effect of Placenta Growth Factor (PIGF) against hypoxia-reoxgyenation and serum-deprivation induced apoptosis in neonatal rat cardiomyocytes" en
dc.type Presentation en

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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