Effects of Elevated Potassium and Mild Acidification on Skeletal Muscle Contractility and Fatigue in Xenopus Laevis

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dc.contributor.author Jensen, Luke
dc.date.accessioned 2011-05-05T19:00:21Z
dc.date.available 2011-05-05T19:00:21Z
dc.date.issued 2011-05-05T19:00:21Z
dc.identifier.uri http://hdl.handle.net/2092/1588
dc.description Mentor: Debora Christensen en_US
dc.description.abstract Skeletal muscle fatigue is typically thought to involve accumulated lactic acid, increased Pi concentration, decreased muscle glycogen, and/or increased levels of oxygen free radicals, but a clear cause of muscle fatigue has remained elusive. Additionally, it is unclear if these factors affect type I (slow oxidative) and type II (A: fast oxidative, B: fast glycolytic) fibers equally. Recent evidence suggests that increasing extracellular K+ concentration reduces the contractile function of isolated rat soleus muscle, while moderately elevated H+ levels provide a protective effect on contractile force. To study the combined effects of mild acidification with elevated K+ on muscle contraction and fatigue, we stimulated isolated Xenopus laevis gastrocnemius muscles to complete exhaustion following 30 minutes of pre-incubation with one of the following solutions: normal amphibian Ringer’s (pH = 7.45), amphibian Ringer’s with elevated (4mM) K+, amphibian Ringer’s with decreased pH (20mM L-lactic acid), and amphibian Ringer’s with both elevated K+ and H+. Following pre-incubation in their respective solutions, muscles were mounted on a force transducer and stimulated with supramaximal stimuli at 60 Hz until completely fatigued. Force of contraction, muscle kinetics (latency, contraction time, and ½-relaxation time), and time to exhaustion were measured. en_US
dc.description.sponsorship Drake University, College of Arts and Sciences en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2011;11
dc.subject Potassium en_US
dc.subject Acidification en_US
dc.subject Xenopus laevis en_US
dc.subject Muscle contraction en_US
dc.title Effects of Elevated Potassium and Mild Acidification on Skeletal Muscle Contractility and Fatigue in Xenopus Laevis en_US
dc.type Presentation en_US

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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