Expression of sFLT-1 and JMJD6 in Cultured Human Trophoblasts

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Show simple item record Hensing, Joe 2011-04-19T16:31:03Z 2011-04-19T16:31:03Z 2011-04-19T16:31:03Z
dc.description Mentor: Ronald Torry en_US
dc.description.abstract Placenta Growth Factor (PlGF) plays an important role in the formation and growth of the placenta. This protein binds two receptors, a membrane bound Flt-1 (mFlt-1) and a soluble Flt-1 (sFlt-1), the latter of which lacks intracellular signaling. These receptors are splice variants of each other and differ in their intracellular region. Although we have shown that hypoxia increases the expression of sFlt -1 in trophoblast, the mechanism for this is not known. Silencing Jumoji domain-containing protein 6 (Jmjd6) expression in endothelial cells increases soluble Flt-1 expression and hypoxia lowers the expression of Jmjd6 in endothelial cells. However, it is not known if Jmjd6 is found in trophoblasts or if it functions in determining the splicing of Flt-1 in these cells. This study sought to optimize an assay to assess Jmjd6 expression in trophoblast, how its expression is affected by hypoxia, and how this correlates to sFlt-1 expression. Trophoblast were isolated from three different normal placentae and cultured for 24 hours under two conditions: normoxia (21% oxygen) and hypoxia (1-2% oxygen). The RNA was harvested, reversed transcribed into cDNA and sFlt-1/Jmjd6 expression was analyzed in duplicate using real-time PCR. The fold changes in sFlt-1 between the normoxic and hypoxic groups were 1.98, 0.75, and 0.88, for the samples. We optimized the PCR of Jmjd6 by changing annealing temperature and concentrations of primers and cDNA. The fold changes in Jmjd6 are still being determined. In conclusion we determined Jmjd6 is expressed in trophoblast but its influence on sFlt-1 expression is unknown. 32 en_US
dc.description.sponsorship Drake University, College of Pharmacy & Health Sciences en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2011;43
dc.subject Trophoblast en_US
dc.subject Placenta en_US
dc.subject RNA en_US
dc.subject Hypoxia (water) en_US
dc.title Expression of sFLT-1 and JMJD6 in Cultured Human Trophoblasts en_US
dc.type Presentation en_US

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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