Expression of Plant Receptor-Like Kinase Leucine Rich Repeat Regions in E. Coli

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Show simple item record Corns, Andrea Heffernen, Emily Hubbard, Ara May Macksey, Paula Pigg, Kelsey Platt, Andrew Stieff, Maria Tucci, Julia 2011-04-19T16:17:16Z 2011-04-19T16:17:16Z 2011-04-19T16:17:16Z
dc.description Mentor: Heidi Sleister en_US
dc.description.abstract Plants respond to external stimuli through signal transduction pathways. An external signal binds the extracellular domain of a transmembrane receptor-like kinase (RLK) protein, and the signal is transmitted inside the cell through the RLK’s kinase domain. Two important plant RLKs are Arabidopsis CLV1 and rice Xa21. CLV1 regulates plant meristem growth and development, and Xa21 provides resistance to the pathogenic bacterium Xanthomonas oryzae. These functions are initiated by a peptide ligand binding a leucine-rich repeat (LRR) region in the extracellular domains of CLV1 and Xa21. Peptide phage display technology will be used to isolate additional peptide ligands that bind CLV1 and Xa21 LRRs. To this end, the DNAs encoding these LRRs are being cloned into vector pSUMO_Kan for soluble expression in E. coli as a fusion with SUMO protein. Fusion proteins will be isolated from E. coli cells by immobilized metal affinity chromatography. en_US
dc.description.sponsorship Drake University, College of Arts & Sciences en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2011;12
dc.subject Arabidopsis en_US
dc.subject Bacteria en_US
dc.subject Leucine en_US
dc.subject Ligands en_US
dc.title Expression of Plant Receptor-Like Kinase Leucine Rich Repeat Regions in E. Coli en_US
dc.type Presentation en_US

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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