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dc.contributor.authorSteger, Ann M.
dc.date.accessioned2011-01-21T19:20:01Z
dc.date.available2011-01-21T19:20:01Z
dc.date.issued1997-09
dc.identifier.other1997 .S32
dc.identifier.urihttp://hdl.handle.net/2092/1477
dc.description41 leavesen_US
dc.description.abstractAn in vitro Isospora suis sporozoite neutralizing antibody assay has been developed in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies present in sera, milk and monoclonal antibody culture fluids by determining both the amount of intracellular sporozoites by immunofluorescence and the cytopathic effect in ST cells. A monoclonal antibody against the apical complex of I. suis sporozoites inhibited the infection of sporozoites in vitro. Hyperimmune sera produced in goats and guinea pigs inhibited sporozoite infection in ST cells. Milk samples from gilts vaccinated with I. suis contained neutralizing antibodies, however the titers did not correlate with protection observed in nursing pigs after challenge.en_US
dc.language.isoen_USen_US
dc.publisherDrake Universityen_US
dc.relation.ispartofseriesDrake University, College of Arts and Sciences;1997
dc.subjectSwine--Cells--Developmenten_US
dc.subjectSwine--Cells--Evaluationen_US
dc.subjectTestis--Cells--Analysisen_US
dc.titleDevelopment and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cellsen_US
dc.typeThesisen_US


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