Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells

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dc.contributor.author Steger, Ann M.
dc.date.accessioned 2011-01-21T19:20:01Z
dc.date.available 2011-01-21T19:20:01Z
dc.date.issued 1997-09
dc.identifier.other 1997 .S32
dc.identifier.uri http://hdl.handle.net/2092/1477
dc.description 41 leaves en_US
dc.description.abstract An in vitro Isospora suis sporozoite neutralizing antibody assay has been developed in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies present in sera, milk and monoclonal antibody culture fluids by determining both the amount of intracellular sporozoites by immunofluorescence and the cytopathic effect in ST cells. A monoclonal antibody against the apical complex of I. suis sporozoites inhibited the infection of sporozoites in vitro. Hyperimmune sera produced in goats and guinea pigs inhibited sporozoite infection in ST cells. Milk samples from gilts vaccinated with I. suis contained neutralizing antibodies, however the titers did not correlate with protection observed in nursing pigs after challenge. en_US
dc.language.iso en_US en_US
dc.publisher Drake University en_US
dc.relation.ispartofseries Drake University, College of Arts and Sciences;1997
dc.subject Swine--Cells--Development en_US
dc.subject Swine--Cells--Evaluation en_US
dc.subject Testis--Cells--Analysis en_US
dc.title Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells en_US
dc.type Thesis en_US


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