Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells

dc.contributor.author	Steger, Ann M.
dc.date.accessioned	2011-01-21T19:20:01Z
dc.date.available	2011-01-21T19:20:01Z
dc.date.issued	1997-09
dc.identifier.other	1997 .S32
dc.identifier.uri	http://hdl.handle.net/2092/1477
dc.description	41 leaves	en_US
dc.description.abstract	An in vitro Isospora suis sporozoite neutralizing antibody assay has been developed in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies present in sera, milk and monoclonal antibody culture fluids by determining both the amount of intracellular sporozoites by immunofluorescence and the cytopathic effect in ST cells. A monoclonal antibody against the apical complex of I. suis sporozoites inhibited the infection of sporozoites in vitro. Hyperimmune sera produced in goats and guinea pigs inhibited sporozoite infection in ST cells. Milk samples from gilts vaccinated with I. suis contained neutralizing antibodies, however the titers did not correlate with protection observed in nursing pigs after challenge.	en_US
dc.language.iso	en_US	en_US
dc.publisher	Drake University	en_US
dc.relation.ispartofseries	Drake University, College of Arts and Sciences;1997
dc.subject	Swine--Cells--Development	en_US
dc.subject	Swine--Cells--Evaluation	en_US
dc.subject	Testis--Cells--Analysis	en_US
dc.title	Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells	en_US
dc.type	Thesis	en_US