Inhibitory Effects of Ketoconazole on the Oxidation of Linoleic Acid Micelles, Phospholipid Liposomes, and Human Low Density Lipoprotein (h-LDL)

dc.contributor.author	Munroe, Melissa E.
dc.date.accessioned	2010-09-15T15:13:01Z
dc.date.available	2010-09-15T15:13:01Z
dc.date.issued	1993-07
dc.identifier.other	1993 .M926
dc.identifier.uri	http://hdl.handle.net/2092/1424
dc.description	36 leaves	en_US
dc.description.abstract	Soybean lipoxygenase (SLO) (500-5000 units/ml), Fe(III)Cl3/ascorbate (5-100microM) and hematin (5-100microM) induced oxidative modification of lipids present in micelles of linoleic acid (0.5 mM), liposomes prepared from phospholipid extracts of bovine brain (2.5 mg/ml), and h-LDL (0.5 mg/ml). Oxidation of the lipid substrates was determined by the formation of thiobarbituric acid reactive substances and conjugated dienes. Free iron (Fe(III)Cl3/ascorbate) was the strongest initiator, followed by hematin and SLO. Ketoconazole (KC) attenuated lipid oxidation in a concentration dependent manner at pharmacologically relevant levels (25-100microM) in all three substrates. Pretreatment of liposomes and h-LDL with KC produced greater attenuation of lipid oxidation by the various initiators in comparison to free KC.	en_US
dc.language.iso	en_US	en_US
dc.publisher	Drake University	en_US
dc.relation.ispartofseries	Drake University, College of Arts and Sciences;1993
dc.subject	Ketoconazole	en_US
dc.subject	Low density lipoproteins	en_US
dc.subject	Micelles	en_US
dc.subject	Liposomes	en_US
dc.title	Inhibitory Effects of Ketoconazole on the Oxidation of Linoleic Acid Micelles, Phospholipid Liposomes, and Human Low Density Lipoprotein (h-LDL)	en_US
dc.type	Thesis	en_US