In Vitro Characterization of Resveratrol-Loaded Pluronic Micelles
Stammet, Michelle M.
MetadataShow full item record
Resveratrol (RES), a polyphenol has shown various biological activities but its use is hindered due to its hydrophobicity and short biological half-life. Biological studies require mega or frequent doses of RES to be effective. Pluronics® F88, F98, F108 and F127 form polymeric micelles & can be used to deliver RES. Micelles were prepared by equilibrium (E) or solvent casting (SC) with either 10 % or 20 % w/w RES loading. Micelles prepared by E of 20% w/w RES showed a correlation with the critical micelle concentrations of the Pluronic® used. Micelles prepared by SC with 10 % w/w RES were stable for 48 hr and approached equilibrium solubility over 12 days. Micelles prepared by SC with 20 % w/w RES were able to fully solubilize RES but approached equilibrium solubility rapidly. Cytotoxicity (IC50) studies in human ovarian cancer cell line, SK-OV3 using RES and all Pluronic® micellar formulations with 10 % w/w RES were done. RES alone had IC50 values of 92.00 ± 17.24 μM RES Pluronic® formulations F88, F98, F108 & F127 had IC50 values of 49.69 ± 9.28 μM, 84.57 ± 7.48 μM, 53.12 ± 11.45 μM and 79.15 ± 21.84 μM respectively, indicating slightly greater potency than RES alone. In conclusion, RES Pluronic® formulations prepared by SC form meta-stable systems of different stabilities based on initial loading. Cytotoxicity studies show all RES formulations are at least as potent as RES alone.
Showing items related by title, author, creator and subject.
Assessing Resveratrol Phuronic F 127 Micelle Stability in the Presence of Plasma Proteins Using Fluorescence Resonance Energy Transfer (FRET) Gleason, Scott; Kalapurayil, Matthew (2010-04-21)Resveratrol (RES) is a polyphenolic compound with potential for chemopreventative and chemotherapeutic uses. However, exploration has been hindered by its short biologic half-life of ~6 minutes. Using polymeric micelles ...
Molecular Analysis of the Effects of Resveratrol and Genistein on Signal Transduction in Cancer Cells Hornick, Madeleine; Kang, Yutong (2011-04-20)The overall objective of our research project is to study the effects of Genistein and Resveratrol on cancer cell proliferation. Resveratrol, a stilbene found in high quantities in red grape skin, and Genistein, an ...
Odorzynski, Melissa; Hoang, Tram B.; Bulochnik, Alex; Dang, Ngoc; Mirafzal, Gholam A.; Almoazen, Hassan (2006-05-04)Purpose: To study the effect of changing structure of benzyl esters on its reactivity in sodium dodecyl sulfate micelles. Methods: Ester molecules with benzyl moiety were either purchased or synthesized. Alkyl groups of ...