Genotoxicity of Genistein : A Molecular Analysis

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Show simple item record Verlinden, Nathan 2010-04-21T13:27:19Z 2010-04-21T13:27:19Z 2010-04-21T13:27:19Z
dc.description Advisors: Craige Wrenn, Pramod B. Mahajan en_US
dc.description.abstract Genistein is a naturally occurring phytoestrogen found in soy products. High intakes of soy products are associated with lower incidences of cancer, especially breast cancer. Genistein may act as a chemopreventative agent by affecting estrogen receptors, inhibiting topoisomerase II causing DNA damage, disrupting proliferative pathways, or by acting as an antioxidant. Research, however, is limited on possible adverse effects of genistein consumption, including gentoxicity. Levels of poly-ADPribose polymerase (PARP) and the phosphorylated histone protein H2AX are increased in the presence of DNA damage, and both were used as markers of genotoxicity in this study. Our project looked at the genotoxic effects of genistein on Sprague-Dawley rats. The rats were treated with 50 mg, 100 mg, or 150 mg of genistein by insertion of genistein pellets into the neck. Our control group consisted of rats with placebo pellets inserted into the neck. Kidney and liver samples were taken from each group and assayed for genotoxicity by SDS-PAGE followed by western blot. Protein levels of both PARP and p-H2AX were indetectable compared to positive controls indicating that no DNA damage was present. Further study is needed to verify the safety of genistein containing products. en_US
dc.description.sponsorship Drake University, Pharmaceutical, Biomedical, and Administrative Science Department en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries DUCURS 2010;41
dc.subject Genetic toxicology--Analysis en_US
dc.subject Soyfoods--Estrogen content--Analysis en_US
dc.subject DNA damage en_US
dc.subject Estrogen--Receptors en_US
dc.title Genotoxicity of Genistein : A Molecular Analysis en_US
dc.type Presentation en_US

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  • DUCURS [196]
    Poster sessions and presentation from the Drake University Conference on Undergraduate Research in the Sciences held each April at Olmsted Center on the Drake campus.

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